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Biochemie
Imunoanalýza
Human Hematopoietic‐Myeloid Leukemia Cell Line (AML14)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Peripheral Blood |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,68 ,FAB M2 Acute Myeloid Leukemia |
| Growth Properties |
Suspension ,Immature myeloid precursors |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 8% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 50 µM 2-Mercaptoethanol (CH045) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/human-hematopoieticmyeloid-leukemia-cell-line-aml14-t8171.html |
The AML14 cell line morphologically appears undifferentiated when grown continuously in suspension culture without the addition of exogenous hematopoietic growth factors. AML14 cells can be induced into eosinophilic differentiation with a combination of IL-3, GM-CSF, and IL-5. Therefore, this cell line is suitable for research aiming to understand the regulation and moelcular mechanisms of eosinophil growth and differentiation. Additional cell lines from this panel: Cat. T8172 – Human Hematopoietic‐Myeloid Leukemia Cell Line (AML14.3D10)
