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Biochemie
Imunoanalýza
Immortalized Human Bone Marrow Mesenchymal Cells – hTERT
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Bone Marrow |
| Organism |
Human (H. sapiens) |
| Donor History |
Not disclosed |
| Growth Properties |
Adherent ,spindle-shaped |
| Growth Conditions | |
| Immortalization Method |
Serial passaging and transduction with recombinant VSV-G retrovirus carrying hTERT and GFP genes |
| Full Information |
https://www.abmgood.com/immortalized-human-bone-marrow-mesenchymal-cells-htert.html |
The Bone Marrow Mesenchymal Cells are important for the growth and differentiation of primitive haematopoietic cells, as well as for maintenance of leukaemic cells. The immortalized cell was created by infecting primary mesenchymal cells with retrovirus containing hTERT and GFP genes separated by IRES under the control of MSCV LTR (pCIneo-hEST2-HA). The Immortalized Bone Marrow Mesenchymal cells can support growth of leukaemic cell lines 380, REH, OP-1 and 697, in addition to support the expansion of CD34+ cells. This cell line is useful not only in supporting the growth of haematopoietic cells, but can also be used in studies concerning bone marrow microenvironment and environmental cues that regulate haematopoiesis.
