Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Porcine Fibroblast Cell Line (iKUF01)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Ear |
| Organism |
Pig (Porcine) |
| Donor History |
PWG micropig |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Transfection with human telomerase reverse transcriptase (hTERT) and subsequent serial passaging. |
| Full Information |
https://www.abmgood.com/Immortalized-Porcine-Fibroblast-Cell-Line-iKUF01-t0390.html |
The iKUF01 cell line is derived from the ear tissue of a PWG pig and was immortalized using human telomerase reverse transcriptase (hTERT). This cell line is characterized by a unique swine leukocyte antigen (SLA) genotype profile that includes multiple alleles across SLA-1, SLA-2, SLA-3, DQA, DQB1, and DRB1, reflecting significant genetic diversity. Its genotype is notable for a mixture of alleles, which contributes to its applicability in studying genetic diversity and immune response variability in swine. This line has been cultured successfully for over 40 passages, maintaining stable growth and morphological characteristics typical of primary fibroblasts.Additional cell lines from this panel:T0390 – Immortalized Porcine Fibroblast Cell Line (iKUF01)T0391 – Immortalized Porcine Fibroblast Cell Line (iKUF02)T0392 – Immortalized Porcine Fibroblast Cell Line (iKUF03)T0393 – Immortalized Porcine Fibroblast Cell Line (iKUF04)T0394 – Immortalized Porcine Fibroblast Cell Line (iKUF07)T0395 – Immortalized Porcine Fibroblast Cell Line (iKUF09)
