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Imunoanalýza
Immortalized Rat Portal Myofibroblasts (RGF)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Liver |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Sprague-Dawley Rat |
| Growth Properties |
Adherent ,spindle-shaped |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Serical passaging and chemical mediated transfection with SV40 large T Antigen mammalian expression vector |
| Full Information |
https://www.abmgood.com/immortalized-rat-portal-myofibroblasts-rgf.html |
Activated primary rat portal fibroblasts (PF) were immortalized with the SV40 large T antigen mammalian expression vector to generate the Immortalized Rat Portal Myofibroblasts (RGF). RGF cells are negative for hepatic stellate cell markers, and like the primary cells, they demonstrate inhibition of cholangiocyte proliferation in co-culture conditions. The easily transfectable nature of these cells make them an ideal research tool for studying both the function of myofibroblasts and their contribution to liver fibrosis progression. abm offers the sorted Immortalized Rat Portal Myofibroblasts (RGF-N2) (T0069).Gene expression analysis conducted to confirm the expression of common liver myofibroblast-associated genes.
