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Immortalized Rat Tracheal Epithelial Cells (SPOC-1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Airway |
| Organism |
Rat (R. norvegicus) |
| Donor History |
10 – 14 weeks old |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow XII Medium (TM012) + 5 ng/ml EGF (Z100139) + 5 µg/ml Transferrin + 5 µg/ml Insulin (TM053) + 0.1 µg/ml Hydrocortisone (TM066) + 0.1 µg/ml Cholera toxin + 50 mM Ethanolamine + 50 mM Phosphoethanolamine + 0.8 mM CaCl2 + 1.5 mg/ml essentially globulin-free bovine serum albumin (Sigma-Aldrich ,A4161) + 0.1 µg/ml Bovine pituitary extract + 15mM HEPES (TM058) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. |
| Immortalization Method |
Spontaneous immortalization |
| Full Information |
https://www.abmgood.com/immortalized-rat-tracheal-epithelial-cells-spoc-1.html |
Mucus hypersecretion is a hallmark of inflammatory airway diseases, but few immortalized tracheal cell lines maintain the ability to differentiate into mucus-producing epithelium. Immortalized Rat Tracheal Epithethelial Cells (SPOC-1) were spontaneously derived from secondary cell cultures. Like other tracheal cell lines, SPOC1 exhibits increased proliferative capacity and decreased growth factor requirements relative to primary cells, but is unique in being non-tumorigenic, having a diploid karyotype and possessing the ability to form gland-like epithelial structures in tracheal grafts, which are comprised of mucin-producing mucous goblet cells. These characteristics position SPOC1 as a valuable tool for the study of airway epithelial secretory cell differentiation, function and neoplastic progression, as well as the key molecular events underlying mucous production.
