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Imunoanalýza
NF-κB Reporter & CD14 Stable Expressing CHO Cell Line (3E10)
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Chinese Hamster (C. griseus) |
| Donor History |
Female ,Adult ,Chinese hamster |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F-12 Medium + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 400 µg/ml Hygromycin (G265) and 10 μg/ml Ciprofloxacin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/nf-b-reporter-cd14-stable-expressing-cho-cell-line-3e10.html |
The CHO/CD14.ELAM.Tac reporter cell line (clone 3E10) expresses inducible membrane CD25 under control of a region from the human E-selectin promoter containing nuclear factor-κB (NF-κB) binding sites; this promoter element is absolutely dependent upon NF-κB.
