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SK-N-AS Stably Overexpresisng THAP1 S21T – Clone 9
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Brain |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,6 ,Caucasian ,Neuroblastoma |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 250 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/SK-N-AS-Stably-Overexpresisng-THAP1-S21T-Clone-9-t3306.html |
This cell line features a stable human neuronal cell line expressing either wild-type or mutates THAP1 proteins. THAP1 (THAP domain-containing apoptosis-assocaited protein 1 ) in implicated in DYT6 dystonia, therefore these cell lines serve as a useful model for studies ralated to THAP1 function in dystonia. THAP1 overexpression results in increased SOD2 protein expression in SK-N-AS cells.
