B16-F10-Luc Cells

B16-F10-Luc is a bioluminescent derivative of the B16-F10 murine melanoma cell line, which was originally established from a spontaneous melanoma arising in a C57BL/6 mouse. The parental B16-F10 line is widely used in cancer research owing to its high metastatic potential and robust, reproducible engraftment in syngeneic C57BL/6 hosts. In B16-F10-Luc cells, a luciferase gene has been stably integrated under the control of a constitutively active promoter (commonly EF-1α or CMV) by lentiviral transduction, resulting in high-level, stable luciferase expression across passages.B16-F10-Luc cells emit bioluminescent signal in the presence of a luciferin substrate, enabling non-invasive, quantitative in vivo bioluminescence imaging (BLI) of tumour growth, dissemination and metastasis in live animals. The line is suitable for both subcutaneous and intravenous (experimental metastasis) models in C57BL/6 mice, with metastatic colonies forming preferentially in the lungs. Luminescence output is linearly proportional to viable cell number, allowing real-time, longitudinal monitoring of tumour burden without sacrifice of animals at each time point. Luciferase expression does not significantly alter the in vitro or in vivo growth kinetics of B16-F10 cells.Key applications include evaluation of antitumour agents, immunotherapies and metastasis-inhibiting compounds in syngeneic models, as well as quantitative pharmacodynamic imaging in preclinical oncology studies.