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Biochemie
Imunoanalýza
3xFLAG-AGO2 Stable Expressing HEK293T Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 5% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/3xflag-ago2-stable-expressing-hek293t-cell-line.html |
MicroRNAs (miRNAs) comprise a class of small non-coding RNAs that regulate the stability and/or translatability of most protein-coding transcripts. They guide AGO proteins within the RNA-induced silencing complex (RISC) and plays a part to degrade or repress the translation of their target mRNAs. 3xFLAG-AGO2 stable expressing HEK293T cells can allow you to investigate the susceptibility of AGO2-loaded miRNA to different classes of nucleases and can be used in complement to in vivo studies to identify new miRNA decay factors.
