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A375 Vemurafenib Resistant Stable Cell line (A375-RL)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Skin |
| Organism |
Human (H. sapiens) |
| Donor History | |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1.5 g/mL Sodium Bicarbonate + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/A375-Vemurafenib-Resistant-Stable-Cell-line-A375-RL-.html |
To obtain the resistant lines, cells were cultured in the presence of vemurafenib an inhibitor of BRAF V600E, starting from a concentration of 0.05 μM and ending at 3.2 μ M for A375. The used drug concentration was doubled every two weeks. After the cells achieved the resistance to the inhibitor, they were maintained in a culture medium with 1 μM of vemurafenib to prevent the loss of resistance. Resistance was confirmed via Western blot analysis.
