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ABCB1(Human) and ABCG2 (Human) Stable Overexpression MDCK Cell Line – Clone #31
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Dog (Canine) |
| Donor History |
Female ,Adult ,Normal ,Cocker Spaniel |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.5 µg/ml Puromycin (G264) and 4.0 mg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
The ABCB1(Human) and ABCG2 (Human) Stable Overexpression MDCK Cell Line is a specialized tool designed for advanced pharmacological and biochemical research. This cell line is derived from MDCK (Madin-Darby Canine Kidney) cells, which have been genetically engineered to stably overexpress human ABCB1 and ABCG2 genes. ABCB1 and ABCG2 are crucial members of the ATP-binding cassette (ABC) transporter family, known for their role in multidrug resistance (MDR) and drug efflux mechanisms. T9814 is suitable for studying the pharmacokinetics and drug interaction dynamics of potential therapeutics, particularly in understanding how drugs are transported across cellular membranes and their efficacy in overcoming multidrug resistance. This cell line is instrumental for researchers focusing on cancer therapy, drug development, and membrane transport biology, providing invaluable insights into drug resistance mechanisms and the development of more effective treatment strategies.
