BHK-21 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Immortalized Cells

Tissue Type

Hamster cell lines

Organism

Golden Hamster

Donor History
Growth Properties

Adherent

Growth Conditions

Culture medium: EMEM (MEM Eagle)

,

w: 2 mM L-Glutamine

,

w: 2.2 g/L NaHCO3

,

w: EBSS (Cytion article number 820100a). Supplements: Supplement the medium with 10% FBS and 1% NEAA. Dissociation reagent: Accutase. Seeding density: 1 to 3 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/BHK-21-Cells/305252

SKU: 305252 Kategorie: ,
Mgr. Otakar Fišer
Máte otázky k tomuto produktu? Kontaktujte našeho specialistu.
Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The BHK-21 cell line is derived from the kidney of a baby hamster (Mesocricetus auratus). These cells are fibroblast-like in morphology and are widely used in various fields of biological and medical research. BHK-21 cells are adherent and grow rapidly in culture, making them an excellent model for studying cell biology, virology, and the production of recombinant proteins. They are especially noted for their use in the propagation of viruses, including foot-and-mouth disease virus, and in the production of viral vaccines.
Researchers utilize the BHK-21 cell line for its robust growth characteristics and its ability to support the replication of a wide range of viruses. This makes it a critical tool in virology research, vaccine production, and gene expression studies. BHK-21 cells are used in genetic engineering and biotechnology for the production of recombinant proteins and monoclonal antibodies.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.