Cathepsin D Stably Over-expressing SH-SY5Y Cell Line

This cell line was developed using the Tet-On gene expression system to enable tetracycline-inducible overexpression of Cathepsin D (CD). The human neuroblastoma SH-SY5Y cells were initially transfected with the pcDNA6/TR© vector and subsequently with vectors containing Cathepsin D cDNA. Stable expression was achieved by selecting with blasticidin, followed by induction with tetracycline. Cells express high levels of enzymatically active Cathepsin D when induced, which is shown to reduce the proliferative potential of the cells, possibly through downregulation of the pro-oncogenic MAPK signaling pathway. Ideal for studying the anti-proliferative effects of Cathepsin D in neuroblastoma, the interaction with EGFR signaling, and exploring new therapeutic approaches involving the manipulation of lysosomal enzymes.Additional cell lines from this panel: T3529 – Cathepsin D Stable Knockout SH-SY5Y Cell Line