CCK-81 Cells

The CCK-81 cell line is a human colorectal adenocarcinoma model derived from a primary tumor. It is commonly used in cancer biology studies focusing on gastrointestinal malignancies and has been characterized for various genetic alterations and drug response profiles. According to functional screening of tumor suppressor genes, CCK-81 expresses mutant **TP53**, as confirmed by yeast-based functional assays, with only about 6% of colonies showing transcriptionally active p53 phenotype, indicating a loss-of-function mutation. This mutation status aligns with its tumorigenic origin and contributes to its relevance as a model for studying p53-deficient colorectal cancers.

CCK-81 has also been included in major cancer cell line compendia such as the Cancer Cell Line Encyclopedia (CCLE), where it has been profiled across multiple omics layers including gene expression, copy number variation, mutation status, and drug sensitivity. Its inclusion in these datasets allows for integrated analyses of pathway dependencies and therapeutic vulnerabilities across colorectal cancer subtypes. For instance, proteogenomic profiling has highlighted that colorectal cancer cell lines, including CCK-81, often exhibit dysregulated signaling pathways such as Wnt/β-catenin and MAPK, making them suitable for precision oncology studies targeting these axes.