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HEK293-GPRC5D Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Transformed cell lines |
| Organism |
Human |
| Donor History |
Transformed/immortalized; non-tumorigenic (HEK293 background) |
| Growth Properties |
Monolayer ,adherent |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS ,1 mM sodium pyruvate ,10 mM HEPES ,1% NEAA. Add Geneticin (G418-Sulfat) to achieve a final concentration of 1 mg/mL. Dissociation reagent: Trypsin-EDTA. Seeding density: 2 to 4 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
HEK293-GPRC5D cells are human embryonic kidney 293 (HEK293) cells engineered to stably express human G protein-coupled receptor family C group 5 member D (GPRC5D), an orphan receptor belonging to the class C G protein-coupled receptor family. GPRC5D exhibits highly restricted expression in normal tissues, with predominant expression reported in hair follicles, keratinized tissues, and plasma cells. Importantly, the receptor is highly expressed in multiple myeloma and certain other plasma cell malignancies, where its limited normal tissue distribution and robust tumor-associated expression have made it a prominent target for immunotherapeutic development, particularly in patients relapsing after BCMA-directed therapies.
HEK293-GPRC5D cells are widely used in hematologic oncology research and therapeutic development for characterization of GPRC5D-targeted monoclonal antibodies, bispecific T-cell engagers, antibody-drug conjugates, and CAR-T or CAR-NK cell therapies. The stable recombinant expression system enables quantitative evaluation of antigen binding affinity, receptor occupancy, surface expression density, epitope specificity, and target-dependent cytotoxicity. These cells are particularly valuable for assessing the activity and selectivity of engineered immune cell therapies and T-cell redirecting biologics targeting GPRC5D-positive malignancies. Additional applications include flow cytometry assay development, reporter assays, high-throughput screening, and validation of receptor-specific imaging agents or diagnostic reagents.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
