Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
HEK293-PSMA Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Transformed cell lines |
| Organism |
Human |
| Donor History |
Transformed/immortalized; non-tumorigenic (HEK293 background) |
| Growth Properties |
Monolayer ,adherent |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS ,1 mM sodium pyruvate ,10 mM HEPES ,1% NEAA. Add Geneticin (G418-Sulfat) to achieve a final concentration of 1 mg/mL. Dissociation reagent: Trypsin-EDTA. Seeding density: 2 to 4 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
HEK293-PSMA cells are human embryonic kidney 293 (HEK293) cells engineered to stably express human prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (FOLH1/GCPII). PSMA is a type II transmembrane glycoprotein with enzymatic folate hydrolase and carboxypeptidase activity that is highly expressed in prostate cancer, particularly in advanced, metastatic, and castration-resistant disease. In addition to prostate malignancies, PSMA expression has also been observed in the neovasculature of various solid tumors. Because of its strong tumor-associated expression and accessible extracellular domain, PSMA has become a major target for diagnostic imaging, radioligand therapy, antibody-based therapeutics, and engineered immune cell approaches.
HEK293-PSMA cells are widely used in oncology research and therapeutic development for characterization of PSMA-targeted monoclonal antibodies, antibody-drug conjugates, radiopharmaceuticals, bispecific T-cell engagers, CAR-T cell therapies, and small-molecule inhibitors. The stable recombinant expression system enables quantitative analysis of ligand binding, receptor occupancy, antigen density, internalization kinetics, and target-dependent cytotoxicity. These cells are particularly valuable for evaluating PSMA-directed imaging probes and radioligand platforms, as PSMA undergoes efficient internalization following ligand engagement. Additional applications include flow cytometry assay development, uptake studies, reporter assays, high-throughput screening, and validation of targeted delivery systems for prostate cancer therapeutics.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
