HGC-27 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Gastric cancer cell lines

Organism

Human

Donor History

Gastric adenocarcinoma

Growth Properties

Monolayer

,

adherent

Growth Conditions

Culture medium: DMEM:Ham's F12 (1:1)

,

w: 3.1 g/L Glucose

,

w: 2.5 mM L-Glutamine

,

w: 15 mM HEPES

,

w: 0.5 mM Sodium pyruvate

,

w: 1.2 g/L NaHCO3 (Cytion article number 820400a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 to 2 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/HGC-27-Cells/300436

SKU: 300436 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

HGC-27 is a human gastric carcinoma cell line derived from the metastatic site of an adult patient. The cell line exhibits an epithelial morphology and is commonly used in the study of gastric cancer pathogenesis and the cellular responses to various chemotherapy agents. HGC-27 cells have been utilized in numerous studies to investigate mechanisms of cancer cell proliferation, apoptosis, and metastasis. They serve as a valuable model for understanding the complex molecular interactions and pathways involved in gastric cancer, including the response to therapeutic compounds and the investigation of novel drug targets.

These cells are also instrumental in studying the role of various genetic and epigenetic modifications in gastric cancer progression. Research using HGC-27 has contributed to insights into cellular processes like epithelial-to-mesenchymal transition (EMT), a critical event in cancer metastasis. Additionally, the cell line has been used to explore receptor signaling pathways and their impact on cancer cell behavior, providing crucial data for the development of targeted therapies. Overall, HGC-27 is an important tool in the advancement of gastric cancer research, helping to pave the way for new therapeutic strategies and improving our understanding of disease mechanisms.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.