HK-CRISPR-Tpr-mEGFP Cells

Unit

3 x 10^6 cells/ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Reporter-labeled cells

Organism

Human

Donor History

Adenocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/HK-CRISPR-Tpr-mEGFP-Cells/300662

Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The HK-CRISPR-Tpr-mEGFP cell line is a specialized model developed for advanced genetic research, particularly in genome editing and gene expression studies. Derived from HeLa Kyoto cells, it integrates CRISPR/Cas9 technology for precise genomic modifications. The incorporation of the mEGFP (monomeric Enhanced Green Fluorescent Protein) reporter gene facilitates real-time visualization and tracking of cellular processes, making it a robust tool for studying gene function, protein localization, and dynamic cellular events in live cells.

This cell line is especially useful for nephrology research, drug discovery, and toxicology studies. The expression of the Tpr gene, a component of the nuclear pore complex, aids in understanding nuclear transport mechanisms and cellular compartmentalization. Researchers use HK-CRISPR-Tpr-mEGFP cells to explore the roles of nuclear pore proteins in various cellular pathways, contributing to insights in cancer, viral infections, and genetic disorders.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.