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Human GRASP55 Stable Knockout HEK293 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,Fetus |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Human-GRASP55-Stable-Knockout-HEK293-Cell-Line-T3442.html |
This cell line features a CRISPR/Cas9 mediated knockout of Grasp55 in HEK293 cells. Genomic sequencing, immunofluorescence and Western blot were used to confirm gene knockdown. Overall, the GRASP knockout cell panel is a useful tool for studying the role of GRASP55 and GRASP65 proteins in other important cellular processes. GRASP55 or GRASP65 single-knockout showed partially impaired Golgi cisternal stacking, whereas double-knockout of both GRASP proteins disassembled the entire Golgi structure.Complete list of cell lines from this panel:Cat. T3442 – Human GRASP55 Stable Knockout HEK293 Cell LineCat. T3443 – Human GRASP65 Stable Knockout HEK293 Cell LineCat. T3444 – Human GRASP55 & GRASP65 Stable Knockout HEK293 Cell LineCat. T3445 – Human GRASP55 Stable Knockout HeLa Cell LineCat. T3446 – Human GRASP65 Stable Knockout HeLa Cell LineCat. T3447 – Human GRASP55 & GRASP65 Stable Knockout HeLa Cell Line
