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Imunoanalýza
Human MPND CRISPR Knockout NCM460 Cell Line – T3-15
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Colon |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,68 ,Hispanic ,Normal tissue (patient with gastric cancer) |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.6 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Human-MPND-CRISPR-Knockout-NCM460-Cell-Line-T3-15-T9820.html |
This cell line features a targeting knockout of Mpr1/Pad1 N-terminal (MPN) domain-containing protein (MPND) in NCM460 cells mediated by CRISPR/Cas9 genome editing technology. This knockout cell line is particularly valuable for investigating MPND’s implications in gastrointestinal physiology or pathology, and it could be pivotal for drug discovery and developmental biology research. The cell line provides a robust platform for studying gene function, cellular responses to environmental factors, and potential therapeutic targets in the context of intestinal health and disease.
