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IER3IP1(Human) CRISPR Knock Out 293T Cell Line-T2-11
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History | |
| Growth Properties | |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.6 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/IER3IP1Human-CRISPR-Knock-Out-293T-Cell-Line-T2-11-T9816.html |
The IER3IP1(Human) CRISPR Knock Out 293T Cell Line-T2-11 is a powerful resource for molecular biology and genetic engineering research. Leveraging the precision of CRISPR-Cas9 technology, this cell line features the targeted knockout of the IER3IP1 gene in 293T cells, a human embryonic kidney cell line known for its high transfection efficiency and rapid growth. The IER3IP1 gene is implicated in stress response pathways and cellular apoptosis, making this cell line particularly useful for studies focused on cellular stress mechanisms, apoptosis regulation, and the pathogenesis of diseases associated with the endoplasmic reticulum stress response. The T2-11 clone ensures specificity and efficiency in gene knockout experiments, facilitating advanced research into gene function, cellular stress responses, and potential therapeutic interventions for diseases linked to IER3IP1 dysfunction.
