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Imunoanalýza
IL-10 Secreting Dendritic Cell Line (MuTu DC1940-IL-10)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Spleen |
| Organism |
Mouse (M. musculus) |
| Donor History |
CD11c:SV40LgT-transgenic C57BL/6 mice |
| Growth Properties |
Adherent ,show dendrites |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow V (TM015) + 8% FBS + 0.00005M Beta-Mercaptoethanol (IMPORTANT cells will not grow without) + 10 mM HEPES + 1% P/S + 0.075% of 7.5% sodium bicarbonate ,37.0°C ,5% CO₂. Must batch test FBS for compatibiltiy with cell line growth. To detach cells ,incubate with cold PBS + 5 mM EDTA for one minute. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/il-10-secreting-dendritic-cell-line-mutu-dc1940-il-10.html |
The MuTu DC1940-IL-10 cell line is derived from murine tumor (MuTu) dendritic cells (DC) expressing IL-10. To generate the IL-10 DC line, IL10 gene was obtained from the cDNA of MuTuDC1s stimulated with CpG and amplified by PCR. DNA fragments were purified and inserted into lentiviral vectors. Transgene expression and protein production was confimed through FACS and ELISA. Cells produce IL-6 and IL12p40 when stimulated with CpG1826.This cell line is a novel stable, and functional DC line model for DC related research.
