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Immortalized Bcl-x Mouse Embryonic Fibroblasts – SV40T
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
11-d-pregnant Bcl-x heterozygous mouse. |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions | |
| Immortalization Method |
Transfection with pCDNA3 plasmid encoding SV40 large T antigen |
| Full Information |
https://www.abmgood.com/immortalized-bcl-x-mouse-embryonic-fibroblasts-sv40t.html |
The Immortalized Bcl-x Mouse Embryonic Fibroblasts – SV40T were derived from primary embryonic cells from an 11-d-pregnant Bcl-x heterozygous mouse. The primary cells were immortalized by transfection with pCDNA3 plasmid encoding SV40 large T antigen. The immortalized cells display similar morphological characteristics with the parental cells. The Immortalized Bcl-x Mouse Embryonic Fibroblasts – SV40T is useful in studies in discovering novel signaling pathways, investigating regulatory networks and immune responses particularly related to the Bcl-xL gene. Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
