Immortalized Mouse Hepatic Stellate Cells – SV40

The Immortalized Mouse Hepatic Stellate Cells – SV40 were derived from C57/Bl6 WT mouse via enzymatic digestion and Percoll density gradient centrifugation. The primary cells were immortalized via transfection of the pCMV Simian Virus 40 Large T antigen and hygromycin B selection. The Immortalized Mouse Hepatic Stellate Cells – SV40 are useful in studies focusing on liver fibrosis since it is the major cell type involved in the formation of scar tissue. The cells are particularly applicable in studies associated with inflammatory responses, as NF-κB and inflammatory cytokines levels have been found to change in the Immortalized Mouse Hepatic Stellate Cells upon LPS stimulation. Characterization by genotype analysis, NF-κB activation assay, cytokine ELISA assays, and 3[H]-thymidine cell proliferation assay.