Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Rat Embryonic Striatal (M26-1F) Cells – tsSV40T
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Sprague-Dawley rat embryo ,Straitum |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂. |
| Immortalization Method |
Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen |
| Full Information |
https://www.abmgood.com/immortalized-rat-embryonic-striatal-m26-1f-cells-tssv40t.html |
Immortalized Rat Embryonic Striatal (M26-1F) Cells – tsSV40T has GABAgeric properties with multiple nucleoli, presence of GAD and MAP-2 antigen, absence of vimentin, and no contact inhibition features. It is a tool which can be used to study GABA uptake and release, neural development and differentiation, and is a good model for Huntington’s disease. The cells grown in permissive temperature (33°C), whereas in non-permissive temperature (39.5°C), the morphology of the cells will become multipolar. SV40 large T antigen is stably expressed at both temperatures compared to the Immortalized Rat Embryonic Striatal (M213-2O) Cells – tsSV40T (Cat. No. T0356). Has GABAgeric properties with multiple nucleoli, presence of GAD and MAP-2 antigen, absence of vimentin, and no contact inhibition features.
