Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Rat Ovarian Granulosa Cells (RGA-1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Ovary |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Female ,21 days old |
| Growth Properties |
Loosely adherent and suspension |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 2.5% FBS + 10 µg/ml low-density lipoprotein + 30 µg/ml high-density lipoprotein + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂ |
| Immortalization Method |
Immortalized with a temperature-sensitive mutant (tsA209) of SV40 |
| Full Information |
https://www.abmgood.com/immortalized-rat-ovarian-granulosa-cells-rga-1.html |
Ovarian granulosa cells play an important physiological role in supporting the development and selection of ovarian follicles by controlling oocyte maturation and by producing the steroid hormones estradiol and progesterone; which are critical for maintenance of the ovarian cycle. The immortalized rat granulosa cells exhibit a temperature-dependent phenotype with respect to morphology as well as growth and the expression of prohibitin. RGA-1 cells will serve as a useful model in studies related to the functional relevance of prohibitin during cell differentiation and apoptosis.
