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Imunoanalýza
Immortalized Rat Portal Myofibroblasts (RGF-N2)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Liver |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Sprague-Dawley Rat |
| Growth Properties |
Adherent ,spindle-shaped |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Serical passaging and chemical mediated transfection with SV40 large T Antigen mammalian expression vector |
| Full Information |
https://www.abmgood.com/immortalized-rat-portal-myofibroblasts-rgf-n2.html |
Activated primary rat portal fibroblasts (PF) sorted via the Ntpdase2/Cd39l1 marker were immortalized with the SV40 large T antigen to generate Immortalized Rat Portal Myofibroblasts (RGF-N2). RGF-N2 cells, like primary PFs, demonstrate inhibition of cholangiocyte proliferation in co-culture conditions. The easily transfectable nature of these cells make them an ideal research tool for studying both the function of myofibroblasts and their contribution to liver fibrosis progression.<br /> <br /> <b>abm</b> also offers the non sorted Immortalized Rat Portal Myofibroblasts (RGF) (<a href=”https://www.abmgood.com/immortalized-rat-portal-myofibroblasts-rgf.html#T0068″>T0068</a>).</p> Gene expression analysis conducted to confirm the expression of common liver myofibroblast-associated genes.
