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Immortalized Trisomy 16 Mouse Neuronal Cells (MTh)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Mouse (M. musculus) |
| Donor History |
Fetal mouse bred from double heterozygous (Rb 2H/RB 32 Lub) males and normal C57BL females |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 2 mM L-glutamine (G275) + 10% FBS + 10% adult bovine serum + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Supplementation of plating medium with UCHT1 rat thyroid cell line-conditioned medium |
| Full Information |
https://www.abmgood.com/immortalized-trisomy-16-mouse-neuronal-cells-mth.html |
The Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) were derived from the spinal cord of a trisomy 16 fetal mouse after 12-16 days of gestation. Trisomy 16 in mice is an animal model for trisomy 21, or Down Syndrome, in humans. Since trisomy 16 animals die in utero , the immortalized Trisomy 16 Mouse Neuronal Cells (MTh) offers an immortalized in vitro model. The fetal mouse was bred from double heterozygous (Rb 2H/RB 32 Lub) males and normal C57BL females. Transformation of the cell line was induced via supplementation with UCHT1 rat thyroid cell line-conditioned medium and was confirmed via the detection of vimentin. RT-PCR found an overexpression of enzyme SOD-1, encoded on mouse chromosome 16 and human chromosome 21, and confirmed the trisomy status of the cell line. Immunohistochemistry found the presence of neurological makers, but the absence of glial markers in the Immortalized Trisomy 16 Mouse Neuronal Cells (MTh). Upon comparison to Immortalized Mouse Neuronal Cells (M4b) (Cat. No. T0674), the Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) were found to have a greater basal [Ca2+] and a greater sensitivity to neurotransmitters. No significant difference in choline uptake was observed between the two cell lines. The Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) can be used as an in vitro model to study neuronal biology and physiology related to Down Syndrome.
