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KLK4-KLKP1 Stable Expressing RWPE-1 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Prostate |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,Caucasian ,54 |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM6391) ,37.0°C ,5% CO₂. Selection with 3.5 μg/ml blasticidin |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/klk4-klkp1-stable-expressing-rwpe-1-cell-line.html |
Pseudogenes have recently been implicated in gene regulation in cancer cells. The KLK4-KLKP1 pseudogene is found in more than 30% of prostate cancer patients and represents a rare case where a non-coding pseudogene is converted into a protein-coding gene. This stable cell line was generated by transducing the immortalized prostate cell line RWPE-1, using lentiviruses expressing the KLK4-KLKP1 gene. The transgene enhances cell proliferation, invasion, and tumour formation, as well as cause changes in gene expression. This cell line is useful for studying the role of KLK4-KLKP1 in prostate cancer development, and its possibility as a therapeutic target in drug screening studies.
