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LKB1 Stable Knockdown Cell Line (H1299-408)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lung |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,Caucasian ,43 ,Lymph node metastasis of the lung |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/LKB1-Stable-Knockdown-Cell-Line-H1299-408-t3311.html |
This cell line features a LKB1 Stable Knockdown Using Lentiviral Short Hairpin RNA. To select for the NSCLC cells that were stably expressing shRNA constructs, cells were plated at subconfluent densities and infected with a cocktail of 1 mL of virus-containing medium, 3 mL of regular medium, and 8 ug/mL polybrene. Selection with 0.5 to 2 ug/mL of puromycin was started 48 h after lentivirus infection. After several weeks of selection (2 wk for H1299 or 4 wk for H1650, H522), monolayers of stably infected pooled clones were harvested for use and cryopreserved
