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Biochemie
Imunoanalýza
miR106b-25 Stable Expressing MCF7 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,69 ,Caucasian ,Adenocarcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.5 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/mir106b-25-stable-expressing-mcf7-cell-line.html |
miR106b-25 Stable Expressing MCF7 Cell Line was established by inserting a vector carrying the miR106b-25 gene and stable selection with the presence of puromycin. miR-106b-25 cluster of miRNA is highly conserved in vertebrates but has been reported in studies to be overexpressed in several cancers. This cell line is useful to study the association of miR-106b-25 and cancer progression.
