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MTTP CRISPR/Cas9 Knockout Huh7 Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,57 ,Asian ,Hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/MTTP-CRISPR/Cas9-Knockout-Huh7-Stable-Cell-Line-T3439.html |
This Huh7 cell line features a stable knockout of microsomal triglyceride transfer protein (MTTP) using CRISPR/Cas9 genome editing. This modification allows for the study of MTTP (Microsomal Triglyceride Transfer Protein) function, which is crucial in the biosynthesis and secretion of very low-density lipoproteins (VLDL). Research applications include studying lipid metabolism disorders, liver function, and the development of treatments for conditions like abetalipoproteinemia.Additional cell lines from this panel:Cat. T3440 – ApoB CRISPR/Cas9 Knockout Huh7 Stable Cell Line
