Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
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Fluorometry
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Izolátory nukleových kyselin
Pipetovací roboty
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Jednokanálové
Multikanálové
Sérologické pipety
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PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
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Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Multiple Myeloma Derived Cell Line (NCU-MM-1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Peripheral Blood |
| Organism |
Human (H. sapiens) |
| Donor History |
Japanese Female ,66 ,Primary plasma cell leukemia |
| Growth Properties |
Immature plasma cell line morphology |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/multiple-myeloma-derived-cell-line-ncu-mm-1.html |
This novel cell line, NCU-MM-1, is dervied from a multiple myeloma pateint and carriess two kinds of chromosomal translocations involving the immunoglobulin light chian (IgL) gene loci without the presence of 14q32 translocations (14q+). Additionally, the Ig lambda locus is juxtaposed to the c-MYC locus at 8q24 resulting in the overexpression of c-Myc protein. On the derivative (11) chromosome, Iq kappa locus was also fused to the chromosome 11q23 locus. The cell line presents the following surface antigens: CD38, CD56, HLA-DR and is negative for any other T,B, and myeloid antigents. NCU-MM-1 cells secrete monoclonal IgG-kappa protein. This cell line is useful for for research regarding the proto-oncogene mappe dto 11q23 and for understanding the moecular origin of MM lacking 14q+.
