Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
NCI-H69AR Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Lung cancer cell lines |
| Organism |
Human |
| Donor History |
Lung small cell carcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 20% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
NCI-H69AR is a multidrug-resistant derivative of the parental small cell lung carcinoma (SCLC) cell line NCI-H69. It was developed through continuous selection in increasing concentrations of chemotherapeutic agents such as doxorubicin. As a result, NCI-H69AR serves as a key model system for investigating mechanisms of acquired drug resistance in SCLC. This cell line retains many of the morphological and biochemical features of its parental line but exhibits profound resistance to several cytotoxic agents, making it especially relevant for studying efflux-mediated resistance pathways.
The primary mechanism of resistance in NCI-H69AR involves overexpression of the multidrug resistance protein P-glycoprotein (P-gp), encoded by the MDR1 gene. P-gp functions as an ATP-dependent efflux pump that reduces intracellular drug accumulation, particularly for anthracyclines, vinca alkaloids, and epipodophyllotoxins. Additionally, NCI-H69AR exhibits altered expression of membrane-associated proteins, including annexin II, which may be associated with changes in calcium signaling and vesicular trafficking-processes implicated in drug resistance and cellular stress response. These phenotypic alterations make NCI-H69AR a valuable model for identifying modulators of drug resistance and for evaluating the efficacy of agents targeting efflux mechanisms or bypassing resistance pathways altogether.
NCI-H69AR has also been used in comparative studies with its parental line to delineate changes in gene and protein expression, drug sensitivity profiles, and response to pharmacologic inhibitors. This comparative framework helps clarify the evolution of drug resistance in cancer and contributes to the design of combination therapies aimed at re-sensitizing resistant tumors. The line is typically cultured in RPMI-1640 medium supplemented with fetal bovine serum and maintained under standard atmospheric conditions. Its robustness and well-characterized resistance phenotype have secured its place in preclinical research on drug resistance in lung cancer.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
