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PPOX-Cys459 Stable Knockout U-2 OS Cell Line (PPOXCys459: C459/1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,15 ,Osteosarcoma |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/PPOX-Cys459-Stable-Knockout-U-2-OS-Cell-Line-PPOXCys459:-C459/1-T3477.html |
This cell lines features a stable knockout of PPOX-Cys459 in human U-2 OS cells , using CRISPR/Cas9 technology. Knocking out the Cys459 locus of the PPOX gene and effectively abolishing protoporphyrinogen oxidase activity creates a model that mirrors aspects of variegate porphyria, presenting with substantial accumulation of protoporphyrinogen IX and decreased heme levels, which in turn impacts the cell’s oxygen consumption rates and alters mitochondrial function. This cell line is pivotal for investigating the molecular and cellular consequences of disrupted heme synthesis, providing a direct link to studying diseases associated with heme biosynthesis defects, including porphyrias, and offering a platform for therapeutic exploration. Complete list of cell lines from this panel:Cat. T3477 – PPOX-Cys459 Stable Knockout U-2 OS Cell Line (PPOXCys459: C459/1)Cat. T3478 – PPOX-Arg38 Stable Knockout U-2 OS Cell Line (PPOXArg38: R38/1)Cat. T3479 – PPOX-Trp227 Stable Knockout U-2 OS Cell Line (PPOXTrp227: W227/1)
