PtK1 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Immortalized Cells

Tissue Type

Potoroo cell lines

Organism

Potoroo

Donor History
Growth Properties

Monolayer

,

adherent

Growth Conditions

Culture medium: RPMI 1640

,

w: 2.0 mM stable Glutamine

,

w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/PtK1-Cells/608393

SKU: 608393 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

PtK1 is a kidney epithelial cell line derived from the rat kangaroo, Potorous tridactylus. Known for its large, flat cells, PtK1 is widely used in microscopy, particularly in studies involving mitosis and chromosome behavior. The large size of its chromosomes makes PtK1 an ideal model for visualizing chromosome dynamics during cell division, making it a popular choice in cytogenetic and molecular biology research. PtK1 cells have also been utilized in studies involving cell fusion and hybridization, particularly between marsupial and eutherian species. These cells are often employed in somatic cell genetics due to their suitability for drug resistance selection. Researchers have developed drug-resistant variants of PtK1, making them useful for isolating hybrid cells and advancing our understanding of chromosome segregation and gene mapping in interspecies hybrids.The cells are positive for keratin by immunoperoxidase staining.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.