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SPRY4-IT1 Stably Expressing Human HT-29 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Colon |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,44 ,Colorectal adenocarcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/spry4-it1-stably-expressing-human-ht-29-cell-line.html |
Long non-coding RNA SPRY4-IT1 shows associate with several different tumors when being up-regulated, it could act as a novel prognostic biomarker for hepatocellular carcinoma, melanoma, breast and prostate cancer cells. Spry4-it1 Stably Expressing Human HT-29 Cell Line would be a useful tool for gene therapy studies as well as tumor gene manipulations.
