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Stable Human ER Positive Breast Epithelial Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Human (H. sapiens) |
| Donor History | |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Mix 3:1 ratio of PriGrow III (TM003) and Ham's F12 media + 5% FBS + 2 mM glutamine (G275) + 0.5 µg/ml hydrocortisone + 5 µg/ml insulin (TM053) + 10 ng/ml cholera toxin + 10 ng/ml epidermal growth factor (Z100139) + 1.8×10⁻⁴ M adenine + 10 µM Y-27632 (Sigma) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-human-er-positive-breast-epithelial-cell-line.html |
Stable Human ER Positive Breast Cells expresses endogenous estrogen receptors (ER) in culture. It is a viable cell culture system enabling end users to conduct studies with oestrogen and screening of drug candidates suitable for breast cancer. The cells are a reliable source of renewable culture as the cells is immortalized with hTERT (pBABE-neo-hTERT vector), thus limits batch to batch variation and gives expression of the estrogen receptors to manipulate.
