SV-HUC-1 Cells

Culture medium: Ham's F12K Medium

w: 2.0 mM L-Glutamine

w: 2.0 mM Sodium pyruvate

w: 2.5 g/L NaHCO3 (Cytion article number 820608a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

or CM-1 (Cytion catalog number 800100)

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

5% CO2

humidified atmosphere.