SW-1463 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Intestine cancer cell lines

Organism

Human

Donor History

Rectal adenocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM:Ham's F12 (1:1)

,

w: 3.1 g/L Glucose

,

w: 2.5 mM L-Glutamine

,

w: 15 mM HEPES

,

w: 0.5 mM Sodium pyruvate

,

w: 1.2 g/L NaHCO3 (Cytion article number 820400a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: TrypLE Express (Life Technologies). Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/SW-1463-Cells/300623

SKU: 300623 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The SW-1463 cell line is derived from a human adenocarcinoma of the rectum. It is part of the extensive SW series of cancer cell lines, which have been characterized for their unique genetic and molecular profiles. SW-1463 is notable for its epithelial morphology and tumorigenic potential in immunocompromised mice. The cell line displays a stable growth pattern under standard culture conditions and has been extensively used in cancer biology and drug development studies.

Genomic profiling of SW-1463 has revealed several mutations associated with oncogenesis, including alterations in the KRAS pathway. This makes the cell line a valuable tool for studying colorectal cancer and testing therapies targeting RAS/RAF/MEK/ERK signaling. Additionally, transcriptomic analyses have highlighted dysregulated expression of genes involved in cell cycle regulation and apoptosis, further emphasizing its utility in cancer research.

SW-1463 has also been integrated into high-throughput drug screening programs, where it has shown diverse responses to chemotherapeutic agents and targeted therapies. These studies provide insights into the mechanisms of drug resistance and sensitivity, aiding in the development of personalized medicine strategies.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.