Type I IFN Receptor Knockout Immortalized Mouse Dendritic Cell Line

As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The MutuDC1IFNR -/- cell line is an immortalized splenic CD8α subset (CD11chigh,B220-,DEC205 ,CD24high,CD11b-) of conventional dendritic cells derived from the 1IFNR knockout CD11c:SV40LgT transgenic mice. Like the Wild-type MutuDC cell line (Cat. No. T0528), the MutuDC1IFNR -/- cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Studies has shown that several genes were found to be differentially negatively (Tnfa and Pdl1) or positively (Ifnb, Il10, and Ccr7) upregulated in the MutuDC1IFNR -/- cells upon stimulation. Together with TLR3 knockout (Cat. T3034) and TLR9 knockout (Cat. T3035) MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8α subset.