WSU-DLCL Cells

PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PriGrow II (TM002) + 20% heat-inactivated FBS + 2mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂.Note: cells are sensitive to post-thaw conditions and take additional time to recover. We recommend supplementing with additional FBS (25-30%) during the first week post-thaw. During the initial growth period (1-2 weeks) centrifuge half the cell suspension and discard supernatant

then combine cells with the leftover cell suspension and transfer to new cutlure vessels. Cells have a tendency to clump in culture. Change media to fresh media every 3 days.