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LIN28B Stable Knockout Human A2780 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,Ovarian endometrioid adenocarcinoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/lin28b-stable-knockout-human-a2780-cell-line.html |
LIN28B Stable Knockout Human A2780 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the LIN28B gene (NM_001004317) using the following guide RNA sequence: TACGGGTAACAGGACCTGGT. LIN28B gene is belongs to the lin-28 family. it is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. This cell line is sequenced verified to be biallelic knockout.
