Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Ham-2 Tumor Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Bile Duct |
| Organism |
Hamster (M. auratus) |
| Donor History |
Male ,7 months ,cholangiocarcinoma |
| Growth Properties |
Adherent ,polygonal (tight; patch-like) |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
This Cholangiocarcinoma (CCA) was isolated from a hamster with CCA induced by O. viverrini metacercariae and N-nitrosodimethylamine. Cells exhibit chromosome hyperploidy and demonstrate slower growth, higher adhesion and migration, and resistance to doxorubicin and 5-fluorouracil (5-FU), compared to the Ham-1 cell line. Subcutaneous transplantation of Ham-2 cells into BALB/c Rag-2/Jak3 double-deficient (BRJ) mice forms mucin-producing cancers resembling human tubular cholangiocarcinoma. Intravenous injection was assocated with metastatic nodules in the lungs and livers and BRJ mice. This cell line is suitable for CAA drug discovery and validation related research.
