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ANG Stable Knockout U2OS Cell Line (U2OS ANG KO5)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,15 ,Osteosarcoma (tibia) |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The ANG Stable Knockout U2OS Cell Line (U2OS ANG KO5) was developed using CRISPR-Cas9 technology to completely knock out the angiogenin (ANG) gene in U2OS cells, a human osteosarcoma cell line. Angiogenin is a ribonuclease involved in angiogenesis and cellular response to stress, particularly in the context of tumor growth and metastasis. The knockout of ANG in U2OS cells provides a valuable model for studying the role of angiogenin in cancer biology, including its involvement in tumorigenesis, cell migration, and angiogenesis. This cell line is particularly useful for exploring potential therapeutic strategies targeting angiogenin in cancer treatment.
