C127I Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Mouse cell lines

Organism

Mouse

Donor History

Carcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/C127I-Cells/400134

SKU: 400134 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The C127I cell line is a murine mammary gland epithelial cell line, commonly used in biomedical research for its ability to synthesize and secrete recombinant proteins. These cells originate from the mammary gland of the BALB/c mouse and are particularly noted for their epithelial morphology and responsiveness to hormones and other growth factors. The C127I cell line has been instrumental in the study of gene expression, signal transduction pathways related to cancer development, and the production of viral vectors for gene therapy.

One of the key features of the C127I cell line is its ability to be easily transfected, making it a valuable tool for the production of recombinant proteins and for gene editing studies. It supports the replication of various murine retroviruses, facilitating the production of stable recombinant lines expressing desirable genes. This characteristic has made C127I cells especially useful in the fields of molecular biology and genetics, where they are often employed to explore the effects of gene overexpression or knockdown in a controlled environment.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.