Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
HPAC Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Pancreas cancer cell lines |
| Organism |
Human |
| Donor History |
Adenocarcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: DMEM:Ham's F12 ,1.2 g/L sodium bicarbonate ,2.5 mM L-glutamine ,15 mM HEPES ,0.5 mM sodium pyruvate (0.002 mg/ml insulin ,0.005 mg/ml transferrin) ITS+ ,40 ng/ml hydrocortisone ,10 ng/ml mouse epidermal growth factor (Fisher Scientific cat# CB-40010). Supplements: Supplement the medium with 5% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The HPAC cell line, derived from human pancreatic ductal adenocarcinoma, serves as an essential model for studying pancreatic cancer’s molecular and cellular characteristics. Known for its utility in evaluating the impact of various chemotherapeutic agents and signaling pathways, HPAC cells exhibit key features typical of pancreatic cancer, including resistance mechanisms. Recent studies involving HPAC have focused on understanding drug resistance, particularly to erlotinib, a tyrosine kinase inhibitor that targets the epidermal growth factor receptor (EGFR). Research has demonstrated that resistance to erlotinib in HPAC cells is associated with significant metabolic alterations, such as changes in phospholipid and amino acid metabolism. Specifically, increased levels of short-chain acylcarnitines and changes in glycerophospholipid profiles have been linked to an elevated metabolic state in erlotinib-resistant HPAC cells.
HPAC cells also express matrix metalloproteinases (MMPs), particularly MT1-MMP, which is crucial for their invasive behavior. The Wnt/β-catenin signaling pathway has been implicated in regulating MMP expression, contributing to the cell’s migration and invasion potential. The application of compounds like matrine has been shown to inhibit HPAC cell migration by downregulating MT1-MMP through the suppression of Wnt/β-catenin signaling. These attributes highlight HPAC as a pivotal cell line for exploring therapeutic interventions aimed at mitigating pancreatic cancer’s aggressive and treatment-resistant nature.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
