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CHO-uPAR Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines ,Reproductive system cancer cell lines ,Transformed cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for uPAR (PLAUR/CD87) surface expression |
| Growth Properties |
Adherent/suspension |
| Growth Conditions |
Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
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CHO-uPAR cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human urokinase-type plasminogen activator receptor (uPAR; PLAUR/CD87), a glycosylphosphatidylinositol (GPI)-anchored cell surface receptor involved in extracellular matrix remodeling, cell adhesion, migration, and tissue invasion. uPAR binds urokinase plasminogen activator (uPA), promoting localized conversion of plasminogen to plasmin and thereby facilitating proteolytic degradation of extracellular matrix components. Elevated uPAR expression is associated with aggressive tumor behavior, metastasis, angiogenesis, and poor clinical prognosis across multiple cancer types, including breast, colorectal, pancreatic, and lung cancers.
CHO-uPAR cells are widely used in cancer biology, drug discovery, and targeted therapeutic development for characterization of uPAR-directed antibodies, peptides, small molecules, radioligands, and engineered immune cell therapies. The stable recombinant expression system supports quantitative analysis of ligand binding, receptor occupancy, uPA-uPAR interaction kinetics, receptor internalization, and downstream signaling events associated with migration and invasion pathways. These cells are also useful for evaluating imaging agents, protease-activated therapeutic systems, and anti-metastatic strategies. In assay development workflows, CHO-uPAR cells are commonly applied in flow cytometry, cell adhesion assays, high-throughput screening, and receptor-specific cytotoxicity studies.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
