NCI-H295R Cells

Culture medium: You can purchase our ready-to-use NCI-H295R Cell Growth Medium (820402) or choose to supplement DMEM:Ham's F12 (1:1)

w: 3.1 g/L Glucose

w: 2.5 mM L-Glutamine

w: 15 mM HEPES

w: 0.5 mM Sodium pyruvate

w: 1.2 g/L NaHCO3 (Cytion article number 820400a) with the below additives. Supplements: Supplement the medium with 5% FBS

0.00625 mg/mL insulin

0.00625 mg/mL transferrin

6.25 ng/mL selenium

1.25 mg/mL bovine serum albumin

0.00535 mg/mL linoleic acid. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

or CM-1 (Cytion catalog number 800100)

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

5% CO2

humidified atmosphere.