STC-1 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Mouse cell lines

Organism

Mouse

Donor History

Neuroendocrine adenoma

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 2 to 4 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/STC-1-Cells/305230

SKU: 305230 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The STC-1 cell line is a widely used model derived from a murine enteroendocrine tumor. These cells are notable for their robust secretion of various gastrointestinal hormones, including cholecystokinin (CCK), secretin, and somatostatin. Due to their hormone-secreting capabilities, STC-1 cells are instrumental in studying gastrointestinal physiology and the regulation of digestive processes. Their response to nutrient stimulation makes them particularly useful for investigating the mechanisms underlying hormone release and action in the gut.
STC-1 cells exhibit characteristics typical of enteroendocrine cells, including the presence of dense core secretory granules and the ability to form polarized monolayers in culture. These features allow researchers to use STC-1 cells for in vitro models of gut hormone secretion and to explore the interactions between enteroendocrine cells and other cell types in the intestinal epithelium. Additionally, their murine origin provides a relevant model for comparative studies between human and rodent gastrointestinal physiology.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.